Journal: Frontiers in Microbiology

Article Title: Assembly and Characterization of a Pathogen Strain Collection for Produce Safety Applications: Pre-growth Conditions Have a Larger Effect on Peroxyacetic Acid Tolerance Than Strain Diversity

doi: 10.3389/fmicb.2019.01223

Figure Lengend Snippet: Final produce-relevant strains included in the collection L. monocytogenes, S. enterica , and E. coli , and surrogate organisms.

Article Snippet: Surrogate strain sequences were integrated into phylogenetic analysis with their associated bacterial group except for E. faecium (ATCC 8459) ( ).

Techniques: Control, Cider Assay

Journal: Frontiers in Microbiology

Article Title: Assembly and Characterization of a Pathogen Strain Collection for Produce Safety Applications: Pre-growth Conditions Have a Larger Effect on Peroxyacetic Acid Tolerance Than Strain Diversity

doi: 10.3389/fmicb.2019.01223

Figure Lengend Snippet: Average log reduction of four S. enterica strains and two surrogate strains (avirulent Salmonella , MHM112 and E. faecium , ATCC8459) when pre-grown under different conditions prior to exposure to 40 ppm PAA for 45 s. Pre-growth conditions are shown on the X -axis and include pre-growth (i) at 21°C (21C), (ii) under reduced water activity (Glycerol), (iii) in minimal medium (M9), (iv) to mid-log phase (Midlog), (v) in 4.5% additional NaCl (NaCl), (vi) at pH 5.0 (pH5) and (vii) to stationary phase (Stationary). When calculating log reduction, values with count zero post-sanitizer treatment were substituted with the detection limit (e.g., 100 CFU/mL). Data represent averages from three trials; the standard deviation of the three trials and number of trials with values below detection limit after PAA exposure are listed in .

Article Snippet: Surrogate strain sequences were integrated into phylogenetic analysis with their associated bacterial group except for E. faecium (ATCC 8459) ( ).

Techniques: Activity Assay, Standard Deviation